Perls Stain Kit (Haematology Version)
CE / IVD
This stain kit is used to demonstrate hemosiderin in bone marrow macrophages and iron within sideroblasts
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK165 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Perls Stain A: Hydrochloric Acid Solution 2%
Perls Stain B: Potassium Ferrocyanide Solution 2%
Counterstain: Neutral Red Solution 0.5%
General Information:
Procedure Time : 30 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Perls A & Perls B 2-8 °C. Neutral Red 15-25 °C
Results:
Nuclei: Red
Ferric Iron: Blue
Protocol:
Preparation of Perls Ferrocyanide Solution:
Perls A 2.5ml, Perls B 2.5ml
Mix immediately before use. Must be freshly prepared for staining. Ammount of A & B solution used
depends on number of slides to be stained.
1. Fix the slides in suitable fixative such as methanol for 10-20 minutes. Leave to air dry
2. Treat slides with Perls ferrocyanide solution for 20 minutes
3. Wash in running tap water
4. Rinse in distilled water
5. Stain with neutral red solution for 30 seconds
6. Wash rapidly in distilled water and blot dry
7. Use usual method and viewing microscopically e.g immersion oil
Hazard Information
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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