Perls Van Gieson Stain Kit (Perls 1867, Van Gieson 1889)

SKU: RRSK60 Categories: , ,

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CE / IVD
This stain kit demonstrates ferric iron, collagen and connective tissue in sections

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Description

100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK603 x 50ml3 x 100ml3 x 250ml3 x 500ml

 

Kit Contains:
Perls Stain Solution A – Hydrochloric Acid Solution 2%
Perls Stain Solution B – Potassium Ferrocyanide Solution 2%
Van Gieson Extra

General Information:
Procedure Time : 30 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:  Store Perls solutions 2-8 °C. Other Van Gieson 15-25 °C

Results: 
Ferric Reactive Iron:   Blue
Collagen:                        Purple/Red
Other Tissues:               Yellow

Protocol:  

Preparation of Perls Ferrocyanide Solution
Perls A 2.5ml Perls B 2.5ml Mix immediately before use. Must be freshly prepared for staining. Amount of A & B solution used depends on number of slides to be stained

1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Treat slides with Perls ferrocyanide solution for 20 minutes
3. Wash in running tap water
4. Rinse in distilled water
5. Stain with Van Gieson solution 2-3 minutes
6. Wash quickly in water and blot dry
7. Dehydrate rapidly, clear and mount

Hazard Information

 

 

 

 

Additional information

Pack

100 Test, 1000 Test, 200 Test, 500 Test