Picro Mallory Trichrome Stain Kit
CE / IVD
This stain kit allows the selective demonstration of muscle, collagen fibres and erythrocytes. This trichrome stain uses 3 dyes of differing molecular size
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK90 | 6 x 50ml | 6 x 100ml | 6 x 250ml | 6 x 500ml |
Kit Contains:
Glacial Acetic Acid 0.5% Aqueous x 2
Saturated Aqueous Picric Acid Solution
Picro Mallory Solution A
Picro Mallory Solution B
Phosphotungstic Acid 1% Solution
General Information:
Procedure Time : 105 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25 °C
Results:
Nuclei: Red
Erythrocytes and Myelin: Yellow
Cytoplasm: Various shades of Red, Pink, Orange
Mucous and Cartilage Matrix: Blue
Collagen: Blue
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Place slides in saturated picric acid solution for 1 hour at 56°C
3. Wash in tap water (3 changes of running water for 2 mins), then distiled water
4. Stain with Picro Mallory Solution A for 2 minutes
5. Rinse in distilled water
6. Differentiate with phosphotungstic acid for 5 mins
7. Rinse in distilled water
8. Stain with Picro Mallory Solution B for 30 minutes
6. Rinse with 0.5% glacial acetic acid to wash off excessive stain
7. Dehydrate, clear and mount
Hazard Information
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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