Picro Mallory Trichrome Stain Kit

SKU: RRSK90 Categories: , ,

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This stain kit allows the selective demonstration of muscle, collagen fibres and erythrocytes. This trichrome stain uses 3 dyes of differing molecular size

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK906 x 50ml6 x 100ml6 x 250ml6 x 500ml


Kit Contains:
Glacial Acetic Acid 0.5% Aqueous x 2
Saturated Aqueous Picric Acid Solution
Picro Mallory Solution A
Picro Mallory Solution B
Phosphotungstic Acid 1% Solution

General Information:
Procedure Time : 105 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:  15-25 °C

Nuclei:                                              Red
Erythrocytes and Myelin:            Yellow
Cytoplasm:                                      Various shades of Red, Pink, Orange
Mucous and Cartilage Matrix:    Blue
Collagen:                                          Blue



1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Place slides in saturated picric acid solution for 1 hour at 56°C
3. Wash in tap water (3 changes of running water for 2 mins), then distiled water
4. Stain with Picro Mallory Solution A for 2 minutes
5. Rinse in distilled water
6. Differentiate with phosphotungstic acid for 5 mins
7. Rinse in distilled water
8. Stain with Picro Mallory Solution B for 30 minutes
6. Rinse with 0.5% glacial acetic acid to wash off excessive stain
7. Dehydrate, clear and mount

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100 Test, 1000 Test, 200 Test, 500 Test