Perls Stain Kit
CE / IVD
This stain kit is used to demonstrate ferric salts in tissue sections. Treatment with an acid ferrocyanide solution will result in the unmasking of ferric iron in the form or hydroxide, Fe(OH)3, by dilute hydrochloric acid. The ferric iron then reacts with a dilute potassium ferrocyanide solution to produce an insoluble blue compound, ferric ferocyanide (Prussian Blue)
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK16 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Perls Stain Solution A – Hydrochloric Acid Solution 2%
Perls Stain Solution B – Potassium Ferrocyanide Solution 2%
Counterstain: Neutral Red Solution 0.1%
General Information:
Procedure Time : 30 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Perls A & Perls B 2-8 °C. Neutral Red 15-25 °C
Results:
Nuclei: Red
Ferric Iron: Blue
Protocol:
Preparation of Perls Ferrocyanide Solution:
Perls A 2.5ml, Perls B 2.5ml
Mix immediately before use. Must be freshly prepared for staining. Amount of A & B solution used depends
on number of slides to be stained
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Rinse well in distilled water
3. Treat slides with Perls ferrocyanide solution for 20 minutes
4. Wash in running tap water
5. Rinse in distilled water
6. Stain with neutral red solution for 15 seconds
7. Wash rapidly in distilled water and blot dry
8. Dehydrate rapidly in alcohols, clear and mount
Hazard Information
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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