PAS Stain Kit for fungal cell walls (modified McManus 1946)
CE / IVD
This stain kit is based upon the cell walls of fungi being rich in polysaccharides which can be converted by oxidation to dialdehydes and demonstrated with Schiff reagent
Description
100 Test | 200 Test | 500 Test | 1000 Test | |
Code | – 100 | -200 | -500 | -1000 |
RRSK157 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Periodic Acid Solution 1%
Light Green 0.2% in 0.2% Acetic Acid
Schiff Reagents (Feulgen)
General Information:
Procedure Time : 35 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Schiff reagent 2-8 °C. Other Reagents 15-25 °C
Results:
Fungal Cell walls: Magenta
Tissue glycogen: Magenta
Background: Pale Green
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise slides with periodic acid solution for 5 minutes
3. Wash well in several changes of distilled water
4. Stain with Schiff reagent for 20 minutes at room temperature
5. Wash slides well in running tap water for 5-10 minutes (sections should macroscopically be pink/magenta in colour)
6. Stain sections in light green solution for 1-2 seconds
7. Wash well in water, differentiate in 0.5% acid alcohol and blue in running water or Scotts tap qater substitute
8. Dehydrate, clear and mount
Hazard Information
Additional information
Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
---|