PAS Stain Kit for fungal cell walls (modified McManus 1946)

SKU: RRSK157 Categories: , ,

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This stain kit is based upon the cell walls of fungi being rich in polysaccharides which can be converted by oxidation to dialdehydes and demonstrated with Schiff reagent

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK1573 x 50ml3 x 100ml3 x 250ml3 x 500ml


Kit Contains:
Periodic Acid Solution 1%
Light Green 0.2% in 0.2% Acetic Acid
Schiff Reagents (Feulgen)

General Information:
Procedure Time : 35 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:    Schiff reagent 2-8 °C. Other Reagents 15-25 °C

Fungal Cell walls:     Magenta
Tissue glycogen:       Magenta
Background:              Pale Green


1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise slides with periodic acid solution for 5 minutes
3. Wash well in several changes of distilled water
4. Stain with Schiff reagent for 20 minutes at room temperature
5. Wash slides well in running tap water for 5-10 minutes (sections should macroscopically be pink/magenta in colour)
6. Stain sections in light green solution for 1-2 seconds
7. Wash well in water, differentiate in 0.5% acid alcohol and blue in running water or Scotts tap qater substitute
8. Dehydrate, clear and mount

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100 Test, 1000 Test, 200 Test, 500 Test