Fields Stain Kit

SKU: RRSK25 Categories: , ,

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This stain kit is used for the detection of malarial parasites in blood films. Fields Stain is one of the original Romanowsky stains using methylene blue in a weak solution of eosin. With some modifications it can be used fairly satisfactorily for the rapid staining of thin blood films

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK253 x 50ml3 x 100ml3 x 250ml3 x 500ml


Kit Contains:
Fields Stain A (Aqueous Methylene Blue Solution A)
Fields Stain B (Aqueous Eosin Y Solution B)
Sorensens Buffer 200 x Concentrate pH 6.8 25ml to make 5L

General Information:
Procedure Time : 35 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:    15-25° C.

Malarial Parasites Blue/Purple

Working Stock Solution
Mix 1 volume of stain B (eosin y) to 4 volumes of distilled water. Take 12 drops of the above diluted stain and add 12 drops of Stain A (methylene blue) and mix together before use


1. Place fixed smear on staining rack and add freshly prepared stain (as above) for 1 minute
2. Rinse in distilled water
3. Differentiate the smear in Sorensens buffer (25ml diluted to 5L with distilled water) pH 6.8 for 5 seconds
4. Rinse in distilled water for 2-3 seconds
5. Stand upright on filter paper to drain and dry and if required dip the dry smear in xylene and mount

Hazard Information

Additional information


100 Test, 1000 Test, 200 Test, 500 Test