Fields Stain Kit
CE / IVD
This stain kit is used for the detection of malarial parasites in blood films. Fields Stain is one of the original Romanowsky stains using methylene blue in a weak solution of eosin. With some modifications it can be used fairly satisfactorily for the rapid staining of thin blood films
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK25 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Fields Stain A (Aqueous Methylene Blue Solution A)
Fields Stain B (Aqueous Eosin Y Solution B)
Sorensens Buffer 200 x Concentrate pH 6.8 25ml to make 5L
General Information:
Procedure Time : 35 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Malarial Parasites Blue/Purple
Working Stock Solution
Mix 1 volume of stain B (eosin y) to 4 volumes of distilled water. Take 12 drops of the above diluted stain and add 12 drops of Stain A (methylene blue) and mix together before use
Protocol:
1. Place fixed smear on staining rack and add freshly prepared stain (as above) for 1 minute
2. Rinse in distilled water
3. Differentiate the smear in Sorensens buffer (25ml diluted to 5L with distilled water) pH 6.8 for 5 seconds
4. Rinse in distilled water for 2-3 seconds
5. Stand upright on filter paper to drain and dry and if required dip the dry smear in xylene and mount
Hazard Information
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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