Gomori Reticulin Stain Kit
CE / IVD
This stain kit is a metal impregnation technique used to demonstrate reticulin fibres providing contrast which enables even the finest of fibres to be seen
Description
| 100 Test | 200 Test | |
| Code | – 100 | -200 |
| RRSK800 | 14 x 50ml | 14 x 100ml |
Kit Contains:
Potassium Hydroxide 10%
Ammonia 33% Solution
Silver Nitrate 10% Solution x 2
Potassium Permanganate 0.5% Solution x 2
Ferric Ammonium Sulphate 2% Solution (iron Alum)
Potassium Metabisulphite 2% Solution
Formaldehyde 10% Solution (unbuffered)
Gold Chloride 0.2% Solution
Oxalic Acid 1% Solution
Sodium Thiosulphate 2% Solution
Van Gieson Extra Stain
Sulphuric Acid 3% Solution
General Information:
Procedure Time : 50 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Silver Nitrate 2-8°C. Other Reagents 15-25° C
Results:
Reticulin fibres: Black
Nuclei: Grey/Black
Collagen: Red
Other Tissue: Yellow
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise the sections in acidified potassium permanganate solution for 5 minutes, wash well in tap water
3. Decolourise in 1% oxalic acid solution for 1 minute, wash in tap water
4. Mordant sections in ferric ammonium sulphate solution for 2 minutes. Wash well with several changes of distilled water
5. Treat with filtered ammoniacal silver solution for 1 minute. Wash well with several changes of distilled water
6. Reduce in 10% formalin solution for 1 minute, check microscopically and if under impregnated repeat steps 8-10. Wash in tap water
7. Tone in 0.2% gold chloride solution for 10 minutes, rinse in tap water
8. Treat sections with 2% potassium metabisulphite solution for 1 minute, rinse in tap water
9. Treat with 2% sodium thiosulphate solution for 1 minute, rinse in tap water
10. Counterstain with Van Gieson for 3 minutes and blot dry
Hazard Information
UN3316
Additional information
| Pack | 100 Test, 200 Test |
|---|
