Alcian Blue Stain Kit (pH2.5)

SKU: RRSK400 Categories: , ,

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This stain kit is a broad spectrum stain for mucins. It demonstrates sialomucins, sulfomucins and proteoglycans. Alcian Blue has been demonstrated to bind and precipitate hyaluronic acid, chondroitin sulphate and heparin from an aqueous solution, but does not precipitate glycogen hence is glycogen negative unlike PAS which is positive

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK4003 x 50ml3 x 100ml3 x 250ml3 x 500ml


Kit Contains:
Alcian Blue 8GX 1% in 3% Acetic Acid
Neutral Red 0.1% Aqueous
Acetic Acid 3% solution

General Information:
Procedure Time : 40-45 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C

Acid mucosubstances, hyaluronic, acid, sialomucins:  Blue / Green
Nuclei/Cytoplasm:                                                                Red


  1. Dewax sections, hydrate through alcohols and rinse in tap water
  2. Stain in 1% Alcian Blue for 30 minutes
  3. Rinse in 3% acetic acid for 10-20 seconds
  4. Wash in running tap water for 5 minutes
  5. Counterstain in 0.5% neutral red for 3 minutes
  6. Rinse quickly in water and blot dry
  7. Dehydrate quickly, clear and mount

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100 Test, 1000 Test, 200 Test, 500 Test