Gomori Trichrome Stain Kit
CE / IVD
This stain kit is used in the demonstration of connective tissue. It is a one step trichrome stain and can be used to differentiate between collagen and smooth muscle fibres
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK70 | 6 x 50ml | 6 x 100ml | 6 x 250ml | 6 x 500ml |
Kit Contains:
Bouins saturated aqueous Solution
Haematoxylin Weigerts A
Haematoxylin Weigerts B
Acid Alcohol 0.5% Solution
Gomori Stain
Glacial Acetic Acid 0.5% Aqueous
General Information:
Procedure Time : 120 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Nuclei: Blue/Black
Cytoplasm, keratin, muscle fibres: Red
Collagen and mucus: Green
Protocol:
1. Prepare Weigert Haematoxylin by mixing equal volumes of solution A & B as required
2. Dewax sections, hydrate through alcohols and rinse in tap water
3. Rinse well in distilled water
4. Place sections in Bouins solution for 1 hour at 56 ° C
5. Remove slides and allow to cool to room temperature, wash well in running tap water until all the yellow colour disappears
6. Rinse well in distilled water
7. Stain nuclei with Weigerts iron haematoxylin for 20 minutes
8. Wash in water and differentiate in 0.5% acid alcohol solution, leaving the nuclei slightly overstained
9. Rinse in running tap water
10. Stain with Gomoris trichrome for 15-20 minutes
11. Rinse sections in 0.5% acetic acid and blot dry
12. Dehydrate, clear and mount
Hazard Information
UN1170
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
