Elastic Van Gieson Stain Kit (Verhoeffs) (EVG)
CE / IVD
This stain kit is useful in demonstrating elastic fibres, collagen and muscle. Elastin has a high affinity for the iron-haematoxylin stain and hence will retain the dye longer than other tissue components which will be decolourised by the differentiation
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK40 | 7 x 50ml | 7 x 100ml | 7 x 250ml | 7 x 500ml |
Kit Contains:
Haematoxylin 5% alcoholic
Ferric Chloride 10% solution x 3
Van Gieson Extra
Lugols Iodine
Sodium Thiosulphate 5% Solution
General Information:
Procedure Time : 45 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Elastic Tissue and Nuclei: Black
Collagen: Red
Other Tissue Elements: Yellow (or according to counterstain)
Working Stock Solution
Solution 1: Add 1.25ml 5% alcoholic haematoxylin to 0.5ml 10% ferric chloride and mix. Add 0.5ml lugols iodine and mix well. Make fresh
Solution 2: Add 5ml of 10% ferric chloride to 20ml of distilled water in a container such as a slide mailer Make fresh every time.
Protocol:
- Dewax sections, hydrate through alcohols and rinse in tap water
- Stain in solution 1 for 30 minutes (save solution until stain is completed)
- Wash in tap water
- Differentiate in solution 2, check microscopically for black fibers on a grey background
- Rinse in water
- Treat with sodium thiosulphate for 1 minute to remove staining due to iodine alone
- Wash in tap water
- Counterstain in Van Gieson Stain for 2 minutes
- Blot to remove excess stain
- Dehydrate rapidly, clear and mount
Hazard Information
UN2924
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
