Elastic Van Gieson Stain Kit (Verhoeffs) (EVG)

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This stain kit is useful in demonstrating elastic fibres, collagen and muscle. Elastin has a high affinity for the iron-haematoxylin stain and hence will retain the dye longer than other tissue components which will be decolourised by the differentiation

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK407 x 50ml7 x 100ml7 x 250ml7 x 500ml


Kit Contains:
Haematoxylin 5% alcoholic
Ferric Chloride 10% solution x 3
Van Gieson Extra
Lugols Iodine
Sodium Thiosulphate 5% Solution

General Information:
Procedure Time : 45 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:    15-25° C.

Elastic Tissue and Nuclei:      Black
Collagen:                                    Red
Other Tissue Elements:           Yellow (or according to counterstain)

Working Stock Solution
Solution 1: Add 1.25ml 5% alcoholic haematoxylin to 0.5ml 10% ferric chloride and mix. Add 0.5ml lugols iodine and mix well. Make fresh
Solution 2: Add 5ml of 10% ferric chloride to 20ml of distilled water in a container such as a slide mailer Make fresh every time.


  1. Dewax sections, hydrate through alcohols and rinse in tap water
  2. Stain in solution 1 for 30 minutes (save solution until stain is completed)
  3. Wash in tap water
  4. Differentiate in solution 2, check microscopically for black fibers on a grey background
  5. Rinse in water
  6. Treat with sodium thiosulphate for 1 minute to remove staining due to iodine alone
  7. Wash in tap water
  8. Counterstain in Van Gieson Stain for 2 minutes
  9. Blot to remove excess stain
  10. Dehydrate rapidly, clear and mount

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Additional information


100 Test, 1000 Test, 200 Test, 500 Test