Cold Kinyoun Stain Kit, Malachite Green
CE / IVD
This stain kit is used for the staining of Mycobacterium Tuberculosis and other acid fast organisms in tissue sections and smears. The walls of Mycobacteria are not readily penetrated so phenol is mixed with the basic fuchsin to overcome this. No heat is required for this method
Description
100 Test | 200 Test | 500 Test | 1000 Test | |
Code | – 100 | -200 | -500 | -1000 |
RRSK7 | 4 x 50ml | 4 x 100ml | 4 x 250ml | 4 x 500ml |
Kit Contains:
Carbol Fuchsin Kinyoun
TB Differentiator x 2
Malachite Green 0.5% Aqueous
General Information:
Procedure Time :15 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C
Results:
Acid fast bacilli (M.tuberculosis): Magenta / red
Cells and background material, other organisms: Green
Protocol:
- Dewax sections, hydrate theough alcohols and rinse in tap For smears flood slides with distilled water
- Flood the slides with carbol fuchsin Kinyoun for 5 minutes
- Wash well in distilled water
- Differentiate with TB differentiator until preparation is colourless or pale pink (until no more dye runs out), approximately 3-5 seconds
- Wash well in distilled water
- Counterstain with malachite green for 1 minute
- Wash well in distilled water
- Dehydrate, clear and mount
Hazard Information
UN1992
Additional information
Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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