AFB (Light Green Counterstain)

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This stain kit is used for the staining of Mycobacterium Tuberculosis and other acid   fast organisms in tissue sections and smears. The walls of Mycobacteria are not readily penetrated so phenol is mixed with the basic fuchsin to overcome this. No heat is required for this method.

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK414 x 50ml4 x 100ml4 x 250ml4 x 500ml


Kit Contains:
Carbol Fuchsin Kinyoun
TB Differentiator x 2
Light Green 0.5% Aqueous

General Information:
Procedure Time : 20 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C

Acid fast bacilli (M.tuberculosis):  Magenta/red
Cells and background material, other organisms: Green


Smears: Prepare smears on cleaned slides and air dry for 5-10 minutes. Fix in absolute ethanol for 10 minutes.
Tissues: A standard formaldehyde based fixative provides satifactory results. Tissues should be processed and embedded in paraffin wax and cut at 5 microns

  1. Dewax sections, hydrate through alcohols and rinse in tap For smears flood slides with distilled water
  2. Flood the slides with carbol fuchsin Kinyoun for 5 minutes
  3. Wash well in distilled water
  4. Differentiate with TB differentiator until preparation is colourless or pale pink (until no more dye runs out), approximately 3-5 seconds
  5. Wash well in distilled water
  6. Counterstain with light green for 1 minute
  7. Wash well in distilled water
  8. Dehydrate rapidly, clear and mount

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Additional information


100 Test, 1000 Test, 200 Test, 500 Test