Congo Red Stain Kit (Highman)

SKU: RRSK10 Categories: , ,

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This stain kit is used for the detection of Amyloid Protein in tissue sections. The use of alkaline alcoholic solvents has the effect of supressing staining of other tissue components whilst enhancing hydrogen bonding hence improving selectivity for amyloid. This method has stable solutions and has a high degree of selectivity

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK103 x 50ml3 x 100ml3 x 250ml3 x 500ml


Kit Contains:
Congo Red in 50% ethanol
Haemalum Mayer
0.2% Potassium Hydroxide in 80% ethanol


General Information:
Procedure Time : 28 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:  15-25°C

Eosinophils, Amyloid, Elastin,Keratin:   Orange/Red
Nuclei:                                                             Blue


  1. Dewax sections, hydrate through alcohols and rinse in tap water
  2. Place in congo red Solution for 15 minutes
  3. Differentiate in potassium hydroxide Solution for 3-10 seconds
  4. Wash in running tap water
  5. Stain nuclei with haemalum Mayer for 5 minutes
  6. Wash well in water
  7. Differentiate in 1% acid alcohol for 2-10 seconds
  8. Wash well in water
  9. Blue in Scotts tap water
  10. Wash well in tap water
  11. Dehydrate rapidly, clear and mount

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100 Test, 1000 Test, 200 Test, 500 Test