Cryptosporidium Stain Kit

SKU: RRSK75 Categories: , ,

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CE / IVD
This stain kit is commonly used for staining oocysts of the Cryptosporidium species in foecal smears. It is also useful to confirm the presence of oocysts of Isospora belli and Cyclospora cayetanensis

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Description

100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK755 x 50ml5 x 100ml5 x 250ml5 x 500ml

 

Kit Contains:
Cryptospridium Fixative
Carbol Fuchsin Kinyoun
Malachite Green 0.5% Aqueous
TB Differentiator x 2

General Information:
Procedure Time : 25 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:  15-25°C

Results: 
Oocysts of Cryptosporidium, parvum:    Magenta / Red
Oocysts of Isospora belli:                           Granular / Red
Oocysts of Cyclospora cayetanensis:      Variable Staining (some are acid fast giving a round hole of glassy wrinkled sphere

Protocol:  

  1. Immerse in the Cryptosporidium Fixative for 3 minutes
  2. Flood the slides with carbol fuchsin Kinyoun for 20 minutes
  3. Wash well in distilled water
  4. Differentiate with TB differentiator until preparation is colourless or pale pink (until no more dye runs out), approximately 10 seconds
  5. Wash well in distilled water
  6. Counterstain with malachite green for 1 minute
  7. Wash well in distilled water
  8. If oil immersion is to be used for visualisation of smears the preparation must be air dried for 20-30 minutes

Hazard Information

 

UN1922

 

 

 

Additional information

Pack

100 Test, 1000 Test, 200 Test, 500 Test