AFB (Methylene Blue Counterstain)
CE / IVD
This stain kit is used for the staining of Mycobacterium Tuberculosis and other acid fast organisms in tissue sections and smears. The walls of Mycobacteria are not readily penetrated so phenol is mixed with the basic fuchsin to overcome this. No heat is required for this method.
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK42 | 4 x 50ml | 4 x 100ml | 4 x 250ml | 4 x 500ml |
Kit Contains:
Carbol Fuchsin Kinyoun
TB Differentiator x 2
Methylene Blue (AFB)
General Information:
Procedure Time : 20 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C
Results:
Acid fast bacilli (M.tuberculosis): Magenta/red
Cells and background material, other organisms: Blue
Protocol:
Smears: Prepare smears on cleaned slides and air dry for 5-10 minutes. Fix in absolute ethanol for 10 minutes
Tissues: A standard formaldehyde based fixative provides satisfactory results. Tissues should be processed and embedded in paraffin wax and cut at 5 microns
- Dewax sections. hydrate through alcohols and rinse in tap For smears flood slides with distilled water
- Flood the slides with carbol fuchsin Kinyoun for 5 minutes
- Wash well in distilled water
- Differentiate with TB differentiator until preparation is colourless or pale pink (until no more dye runs out), approximately 3-5 seconds
- Wash well in distilled water
- Counterstain with methylene blue (AFB) for 15 seconds
- Wash well in distilled water
- Dehydrate, clear and mount
Hazard Information
UN1992
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
