AFB (Methylene Blue Counterstain)

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This stain kit is used for the staining of Mycobacterium Tuberculosis and other acid   fast organisms in tissue sections and smears. The walls of Mycobacteria are not readily penetrated so phenol is mixed with the basic fuchsin to overcome this. No heat is required for this method.

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK424 x 50ml4 x 100ml4 x 250ml4 x 500ml


Kit Contains:
Carbol Fuchsin Kinyoun
TB Differentiator x 2
Methylene Blue (AFB)

General Information:
Procedure Time : 20 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C

Acid fast bacilli (M.tuberculosis):  Magenta/red
Cells and background material, other organisms: Blue


Smears: Prepare smears on cleaned slides and air dry for 5-10 minutes. Fix in absolute ethanol for 10 minutes
Tissues: A standard formaldehyde based fixative provides satisfactory results. Tissues should be processed and embedded in paraffin wax and cut at 5 microns

    1. Dewax sections. hydrate through alcohols and rinse in tap For smears flood slides with distilled water
    2. Flood the slides with carbol fuchsin Kinyoun for 5 minutes
    3. Wash well in distilled water
    4. Differentiate with TB differentiator until preparation is colourless or pale pink (until no more dye runs out), approximately 3-5 seconds
    5. Wash well in distilled water
    6. Counterstain with methylene blue (AFB) for 15 seconds
    7. Wash well in distilled water
    8. Dehydrate, clear and mount

Hazard Information






Additional information


100 Test, 1000 Test, 200 Test, 500 Test