Congo Red Stain Kit (Puchtler & Sweat)
CE / IVD
This stain kit is used for the detection of Amyloid Protein in tissue sections. This method removes the need for a differentiation step with the use of a highly concentrated sodium chloride solution which reduces background staining whilst enhancing hydrogen bonding of Congo Red to Amyloid resulting in a progressive staining and highly selective technique
Description
| 100 Test | 200 Test | |
| Code | – 100 | -200 |
| RRSK9 | 12 x 50ml | 12 x 100ml |
Kit Contains:
Saturated Sodium Chloride Alcoholic Solution x 5
Sodium Hydroxide 1% Aqueous Solution
Congo Red in 80% Ethanol Saturated x 5
Haemalum Mayer
General Information:
Procedure Time : 60 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25°C
Results:
Eosinophils, Amyloid, Elastin,Keratin: Red
Various glycoproteins, early lipofuchsins: Magenta
Nuclei: Blue
Protocol:
Firstly 2 separate working solutions must be prepared
Working Solution A Add 0.25ml sodium hydroxide to 25ml alcoholic sodium chloride. Use promptly Working Solution B Add 0.25ml sodium hydroxide solution to 25ml congo red solution. Use promptly
- Dewax sections, hydrate through alcohols and rinse in tap water
- Stain nuclei with Mayers haemalum for 5 mins
- Wash well in tap water
- Differentiate with 1% acid alcohol 2-10 secs
- Wash well in water
- Blue in scotts tap water
- Wash well in water
- Immerse in working solution A for 20 Drain
- Immerse in congo red working solution B for 20 minutes
- Rinse briefly in alcohol (80%)
- Dehydrate rapidly, clear and mount
Hazard Information
UN1170
Additional information
| Pack | 100 Test, 200 Test |
|---|
