Elastic Van Gieson Stain Kit (Miller) (EVG)
CE / IVD
This stain kit uses a combined technique to demonstrate elastic fibres, collagen and muscle. Elastic fibres, especially fine fibres will stain intensely blue with the Miller stain. Elastin and pre elastin fibres are highly cross linked disulphide bridges which require oxidation with potassium permanganate followed by a bleach with oxalic acid
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK11 | 6 x 50ml | 6 x 100ml | 6 x 250ml | 6 x 500ml |
Kit Contains:
Potassium Permanganate 0.5% Solution x 2
Millers Elastin Stain
Sulphuric Acid 3% Solution
Van Gieson Extra Stain
Oxalic Acid 1% Solution
General Information:
Procedure Time : 195 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Elastic fibres: Blue-Black
Mature Collagen: Red
Other tissues e.g. muscle and red blood cells : Yellow
Protocol:
Preparation of Acidified Potassium Permanganate
Mix 9.5ml of potassium permanganate solution with 0.5ml of sulphuric acid solution. This purple solution will keep for 3-4 weeks but should be discarded after using 5 times or if it turns brown
- Dewax sections, hydrate through alcohols and rinse in tap water
- Treat with acidified potassium permanganate for 5 minutes
- Wash well in water and bleach with oxalic acid for 1 minute
- Wash well in water
- Rinse in 95% alcohol (not supplied)
- Stain in Millers elastin stain for 3 hours at room temperature
- Rinse briefly in 95% alcohol to remove excess stain
- Wash well in water
- Counterstain with Van Gieson stain for 2 minutes
- Blot Dry
- Dehydrate rapidly, clear and mount
Hazard Information
UN1170
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
