DPAS Stain Kit
CE / IVD
This stain kit is used to differentiate glycogen from other PAS-positive elements in tissue sections. Glycogen is removed by enzyme digestion. It is often used in conjunction with the PAS kit
Description
100 Test | 200 Test | 500 Test | 1000 Test | |
Code | – 100 | -200 | -500 | -1000 |
RRSK150 | 4 x 50ml | 4 x 100ml | 4 x 250ml | 4 x 500ml |
Kit Contains:
Lipase from porcine pancreas
Periodic Acid Solution 1%
Schiff reagents (Feulgen)
Haemalum Mayer
General Information:
Procedure Time : 75 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Schiff reagent & lipase 2-8° C. Other Reagents 15-25° C.
Results:
Nuclei: Blue-Black
Glycogen: No Magenta staining
Neutral/sialomucins: Magenta
Various glycoproteins, early lipofuchsins: Magenta
Protocol:
- Dewax sections, hydrate through alcohols and rinse in tap water
- Treat with a 1% solution of lipase for 20 minutes at 37°C and then wash well in water
- Oxidise slides with periodic acid solution for 5 minutes
- Wash well in several changes of distilled water
- Stain with schiff reagent for 20 minutes at room temperature
- Wash slides well in running tap water for 5-10 minutes (sections should macroscopically be pink/ magenta in colour)
- Stain sections in haemalum Mayer for 1 minute
- Wash well in water, differentiate in 0.5% acid alcohol and blue in running water or Scotts tap water substitute
- Dehydrate rapidly, clear and mount
Hazard Information
Additional information
Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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