DPAS Stain Kit

Login to See Prices

CE / IVD
This stain kit is used to differentiate glycogen from other PAS-positive elements in tissue sections. Glycogen is removed by enzyme digestion. It is often used in conjunction with the PAS kit

Add to Wishlist
Add to Wishlist

Description

100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK1504 x 50ml4 x 100ml4 x 250ml4 x 500ml

 

Kit Contains:
Lipase from porcine pancreas
Periodic Acid Solution 1%
Schiff reagents (Feulgen)
Haemalum Mayer

General Information:
Procedure Time : 75 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:    Schiff reagent & lipase 2-8° C. Other Reagents 15-25° C.

Results: 
Nuclei:        Blue-Black
Glycogen:   No Magenta staining
Neutral/sialomucins: Magenta
Various glycoproteins, early lipofuchsins:  Magenta

Protocol:  

  1. Dewax sections, hydrate through alcohols and rinse in tap water
  2. Treat with a 1% solution of lipase for 20 minutes at 37°C and then wash well in water
  3. Oxidise slides with periodic acid solution for 5 minutes
  4. Wash well in several changes of distilled water
  5. Stain with schiff reagent for 20 minutes at room temperature
  6. Wash slides well in running tap water for 5-10 minutes (sections should macroscopically be pink/ magenta in colour)
  7. Stain sections in haemalum Mayer for 1 minute
  8. Wash well in water, differentiate in 0.5% acid alcohol and blue in running water or Scotts tap water substitute
  9. Dehydrate rapidly, clear and mount

Hazard Information

 

 

 

 

Additional information

Pack

100 Test, 1000 Test, 200 Test, 500 Test