PAS Stain Kit for fungal cell walls (modified McManus 1946)
CE / IVD
This stain kit is based upon the cell walls of fungi being rich in polysaccharides which can be converted by oxidation to dialdehydes and demonstrated with Schiff reagent
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK157 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Periodic Acid Solution 1%
Light Green 0.2% in 0.2% Acetic Acid
Schiff Reagents (Feulgen)
General Information:
Procedure Time : 35 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Schiff reagent 2-8 °C. Other Reagents 15-25 °C
Results:
Fungal Cell walls: Magenta
Tissue glycogen: Magenta
Background: Pale Green
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise slides with periodic acid solution for 5 minutes
3. Wash well in several changes of distilled water
4. Stain with Schiff reagent for 20 minutes at room temperature
5. Wash slides well in running tap water for 5-10 minutes (sections should macroscopically be pink/magenta in colour)
6. Stain sections in light green solution for 1-2 seconds
7. Wash well in water, differentiate in 0.5% acid alcohol and blue in running water or Scotts tap qater substitute
8. Dehydrate, clear and mount
Hazard Information
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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