Kleihauer Stain Kit
CE / IVD
This stain kit is used to detect foetal cells in a maternal circulation and is based upon the ability of foetal cells to withstand acid pH whereas adult red cells undergo elution of the haemoglobin
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK33 | 8 x 50ml | 8 x 100ml | 8 x 250ml | 8 x 500ml |
Kit Contains:
Kleihauer Buffer Solution 1 x 3
Kleihauer Buffer Solution 2 x 2
Erythrosin 0.1% Acidified Solution
Haemalum Mayer
Denatured Ethanol 80%
General Information:
Procedure Time : 45 minutes (post warming of working solution) (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Foetal cells: Reddish brown
Adult cells: Pale pink ghost outlines
Neutrophils: Appear differentiated as in haematology films
Lymphocytes: Grey/Pink
Protocol:
1. Following preparation, allow blood smears to air-dry for 10-60 minutes
2. Place the dry blood smears in a coplin jar containing the denatured ethanol 80% for approximately 5 minutes.
3. Rinse in distilled water and allow to air dry
4. Place the slides in the Kleihauer buffer solution (pre heated to 37° C in a Coplin jar) for 5 minutes. Agitate the slides at 2 minute intervals
5. Rinse with distilled water and air dry
6. Stain with the haemalum Mayer for 3 minutes
7. Rinse with distilled water
8. Stain with the erythrosin solution for 4 minutes
9. Rinse with distilled water and allow to air dry
10. Slides must be examined using oil immersion
Hazard Information
UN1170
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
