Von Kossa Stain Kit (Strong)

SKU: RRSK39 Categories: , ,

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This stain kit is used to visualise calcium deposits in tissue sections. The sections are treated with a silver nitrate solution and silver is deposited by replacing the calcium reduced by strong light which can then be visualised as metallic silver

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK393 x 50ml3 x 100ml3 x 250ml3 x 500ml


Kit Contains:
Silver Nitrate 10% solution
Sodium Thiosulphate 5% solution
Neutral Red (0.5% Aqueous) solution

General Information:
Procedure Time :  75 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Silver Nitrate 2-8 °C. Other Reagents 15-25 °C

Calcium Salts:   Black
Nuclei:                Red
Cytoplasm:         Pink

1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Place in silver nitrate solution and expose to strong light for 1 hour
3. Wash in 3 changes of distilled water
4. Treat with sodium thiosulphate for 5 minutes
5. Rinse well in distilled water.
6. Counterstain with neutral red for 5 minutes
7. Rinse in water
8. Dehydrate rapidly through alcohols, clear and mount

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100 Test, 1000 Test, 200 Test, 500 Test