Sudan Black B Stain Kit (Histology Version)
CE / IVD
This stain kit is used for staining phospholipids as well as neutral fats. sudan black B does not stain cholesterol, lecithins and free fatty acids. There are two distinct fractions in this dye, the first stains neutral fats blue-black, whilst the second fraction (a basic dye) stains phospholipids grey. Sudan black B does not stain cholesterol, leathiris and free fatty acids
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK17 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Sudan Black Stain in 70% Ethanol
Ethanol 70% solution
Neutral red 0.5% aqueous
General Information:
Procedure Time : 130 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25 °C
Results:
Nuclei: Red
Phospholipids: Grey
Unsaturated Esthers/ Triglicerides: Blue / Black
Protocol:
1. Rinse cryostat sections in 70% ethanol
2. Stain for 2 hours in sudan black B solution
3. Rinse sections in 70% ethanol to remove excess dye for 2-3 minutes
4. Wash in tap water for 2 minutes
5. Blot dry and counterstain in neutral red solution for 2-3 minutes
6. Wash well in water
7. Mount in aqueous mountant or examine under immersion oil
Hazard Information
UN1170
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
