TB Fluorescent Stain Kit I (Auramine Phenol Lempert)

SKU: RRSK18 Categories: , ,

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This stain kit is used for the demonstration of M-Tuberculosis and other acid fast organisms in tissue sections and smears. The protocol uses auramine O which is a yellow cationic dye used as a fluorochrome which attaches to the tubercle bacilli and is visualised by use of fluorescent microscopy

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK183 x 50ml3 x 100ml3 x 250ml3 x 500ml


Kit Contains:
Auramine Phenol Lempert Solution
Auramine Phenol Differentiator
Potassium Permanganate 0.5% Aqueous Solution

General Information:
Procedure Time :  30 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:  15-25 °C

Mycobacteria:    Golden Yellow Rods
Background:      Dark Green


1. Dewax sections hydrate through alcohol and rinse in water. For smears rinse in distilled water
2. Stain slides with auramine phenol lempert for 15 minutes
3. Rinse well in distilled water
4. Differentiate in auramine differentiator for 5 minutes
5. Wash well in distilled water
6. Treat with 0.5% potassium permanganate solution for 30 seconds
7. Wash well in distilled water and drain slides
8. Dehydrate rapidly through alcohols, clear and mount


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