TB Fluorescent Stain Kit II

SKU: RRSK19 Categories: , ,

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This stain kit is used for the demonstration of M-Tuberculosis and other acid fast organisms in tissue sections and smears. The protocol uses auramine O which is a yellow cationic dye used as a fluorochrome which attaches to the tubercle bacilli and is visualised by use of fluorescent microscopy

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK193 x 50ml3 x 100ml3 x 250ml3 x 500ml


Kit Contains:
Auramine Phenol Lempert Solution
Auramine Phenol Differentiator
Potassium Permanganate 0.5% Aqueous Solution

General Information:
Procedure Time :  30 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:  15-25 °C

Mycobacteria:    Golden Yellow Rods
Background:      Dark Green


1. Dewax sections, hydrate through alcohol and rinse in water. For smears rinse in distilled water
2. Preheat the auramine rhodamine to 60 degrees C in an oven or water bath. Mix well after heating and filter onto slides, stain for 10 minutes
3. Rinse well in distilled water
4. Differentiate in auramine differentiator for 5 minutes
5. Wash well in distilled water
6. Treat with 0.5% potassium permanganate solution for 30 seconds
7. Wash well in distilled water and drain slides
8. Dehydrate rapidly through alcohols, clear and mount

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