TB Fluorescent Stain Kit II
CE / IVD
This stain kit is used for the demonstration of M-Tuberculosis and other acid fast organisms in tissue sections and smears. The protocol uses auramine O which is a yellow cationic dye used as a fluorochrome which attaches to the tubercle bacilli and is visualised by use of fluorescent microscopy
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK19 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Auramine Phenol Lempert Solution
Auramine Phenol Differentiator
Potassium Permanganate 0.5% Aqueous Solution
General Information:
Procedure Time : 30 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25 °C
Results:
Mycobacteria: Golden Yellow Rods
Background: Dark Green
Protocol:
1. Dewax sections, hydrate through alcohol and rinse in water. For smears rinse in distilled water
2. Preheat the auramine rhodamine to 60 degrees C in an oven or water bath. Mix well after heating and filter onto slides, stain for 10 minutes
3. Rinse well in distilled water
4. Differentiate in auramine differentiator for 5 minutes
5. Wash well in distilled water
6. Treat with 0.5% potassium permanganate solution for 30 seconds
7. Wash well in distilled water and drain slides
8. Dehydrate rapidly through alcohols, clear and mount
Hazard Information
UN1992
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
