Toluidine Blue Stain Kit

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This stain kit is used for the demonstration of mast cells in tissue sections. The granules within the cytoplasm of mast cells are metachromatic. The metachromatic staining is due to pH, dye concentration and temperature of the basic dye

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK6096 x 50ml6 x 100ml6 x 250ml6 x 500ml


Kit Contains:
Toluidine Blue 1% in 70% alcohol
Sodium Chloride 0.9% aqueous x 5

General Information:
Procedure Time :  12 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:  15-25 °C

Mast Cells:    Violet
Background:  Shades of Blue

Working Solution
Toluidine blue stock 1ml
Sodium chloride solution 9ml
Make Fresh, discard after use

1. Dewax sections, hydrate through alcohol and rinse in tap water
2. Stain with the toluidine blue working solution for 3 minutes
3. Wash with 3 changes of distilled water
4. Dehydrate rapidly through alcohols, clear and mount

Hazard Information





Additional information


100 Test, 200 Test