Von Kossa Stain Kit
CE / IVD
This stain kit is used to visualise calcium deposits in tissue sections. The sections are treated with a silver nitrate solution and silver is deposited by replacing the calcium reduced by strong light which can then be visualized as metallic silver
Description
100 Test | 200 Test | 500 Test | 1000 Test | |
Code | – 100 | -200 | -500 | -1000 |
RRSK38 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Silver Nitrate 5% solution
Sodium Thiosulphate 5% solution
Neutral Red (0.5% Aqueous) solution
General Information:
Procedure Time : 75 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Silver Nitrate 2-8 °C. Other Reagents 15-25 °C
Results:
Calcium Salts: Black
Nuclei: Red
Cytoplasm: Pink
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Place in silver nitrate solution and expose to strong light for 1 hour
3. Wash in 3 changes of distilled water
4. Treat with sodium thiosulphate for 5 minutes
5. Rinse well in distilled water
6. Counterstain with neutral red for 5 minutes
7. Rinse in water
8. Dehydrate rapidly through alcohols, clear and mount
Hazard Information
UN3082
Additional information
Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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