Von Kossa Stain Kit

SKU: RRSK38 Categories: , ,

Login to See Prices

CE / IVD 
This stain kit is used to visualise calcium deposits in tissue sections. The sections are treated with a silver nitrate solution and silver is deposited by replacing the calcium reduced by strong light which can then be visualized as metallic silver

Add to Wishlist
Add to Wishlist

Description

100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK383 x 50ml3 x 100ml3 x 250ml3 x 500ml

 

Kit Contains:
Silver Nitrate 5% solution
Sodium Thiosulphate 5% solution
Neutral Red (0.5% Aqueous) solution

General Information:
Procedure Time :  75 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Silver Nitrate 2-8 °C. Other Reagents 15-25 °C

Results: 
Calcium Salts:   Black
Nuclei:                Red
Cytoplasm:         Pink

Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Place in silver nitrate solution and expose to strong light for 1 hour
3. Wash in 3 changes of distilled water
4. Treat with sodium thiosulphate for 5 minutes
5. Rinse well in distilled water
6. Counterstain with neutral red for 5 minutes
7. Rinse in water
8. Dehydrate rapidly through alcohols, clear and mount

Hazard Information

UN3082

 

 

 

 

Additional information

Pack

100 Test, 1000 Test, 200 Test, 500 Test