PAS Stain Kit (modified McManus 1946)
CE / IVD
This stain kit is used for the demonstration of neutral mucins, glycogen and some early lipofuchsins. The technique is based upon the reactivity of free aldehyde groups within carbohydrates whereby the Schiff reagent forms a bright red magenta end product
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK15 | 3 x 50ml | 3 x 100ml | 3 x 250ml | 3 x 500ml |
Kit Contains:
Periodic Acid Solution 1%
Haematoxylin Harris
Schiff Reagents (Feulgen)
General Information:
Procedure Time : 45 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Schiff Reagent 2-8 °C. Other Reagents 15-25 °C
Results:
Nuclei: Blue-Black
Glycogen: Magenta
Neutral/sialomucins: Magenta
Various glycoproteins, early lipofuchsins: Magenta
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise slides with periodic acid solution for 5 minutes
3. Wash well in several changes of distilled water
4. Stain with Schiff reagent for 20 minutes at room temperature
5. Wash slides well in running tap water for 5-10 minutes (sections should macroscopically be pink/magenta in colour)
6. Stain sections in haematoxylin for 1 minute
7. Wash well in water, differentiate in 0.5% acid alcohol and blue in running water or Scotts tap water substitute
8. Dehydrate, clear and mount
Hazard Information
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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