PAS Stain Kit (modified McManus 1946)

SKU: RRSK15 Categories: , ,

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This stain kit is used for the demonstration of neutral mucins, glycogen and some early lipofuchsins. The technique is based upon the reactivity of free aldehyde groups within carbohydrates whereby the Schiff reagent forms a bright red magenta end product

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK153 x 50ml3 x 100ml3 x 250ml3 x 500ml


Kit Contains:
Periodic Acid Solution 1%
Haematoxylin Harris
Schiff Reagents (Feulgen)

General Information:
Procedure Time : 45 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:  Schiff Reagent 2-8 °C. Other Reagents 15-25 °C

Nuclei:                                                                            Blue-Black
Glycogen:                                                                       Magenta
Neutral/sialomucins:                                                  Magenta
Various glycoproteins, early lipofuchsins:              Magenta

1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise slides with periodic acid solution for 5 minutes
3. Wash well in several changes of distilled water
4. Stain with Schiff reagent for 20 minutes at room temperature
5. Wash slides well in running tap water for 5-10 minutes (sections should macroscopically be pink/magenta in colour)
6. Stain sections in haematoxylin for 1 minute
7. Wash well in water, differentiate in 0.5% acid alcohol and blue in running water or Scotts tap water substitute
8. Dehydrate, clear and mount

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100 Test, 1000 Test, 200 Test, 500 Test