Masson Trichrome (Methyl Blue) Stain Kit
CE / IVD
This stain kit is used to demonstrate connective tissues. Three dyes are used, each with different selectivity for muscle, collagen fibres and erythrocytes hence the term ‘trichrome’ staining
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK20 | 5 x 50ml | 5 x 100ml | 5 x 250ml | 5 x 500ml |
Kit Contains:
Haematoxylin Weigerts (Solution A)
Haematoxylin Weigerts (Solution B)
Phosphotungstic Acid 1% Solution
Ponceau Fuchsin Masson Solution
2% Methyl Blue in 2.5% Acetic Acid
General Information:
Procedure Time : 50 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Nuclei: Blue / Black
Cytoplasm, Neuroglia fibres and Muscle: Red
Collagen and Mucus: Green
Protocol:
1. Prepare Weigert haematoxylin by mixing equal volumes of solution A & B as required
2. Dewax sections, hydrate through alcohols and rinse in tap water
3. Stain nuclei with Weigert’s iron haematoxylin for 20 minutes, wash quickly in water and differentiate in 1% acid alcohol solution (RRSP175 – not supplied), leaving the nuclei slightly overstained
4. Rinse and blue in water
5. Rinse and stain with ponceau fuchsin solution for 5 minutes. Rinse in deionised water
6. Differentiate and mordant in phosphotungstic acid for 15 minutes
7. Transfer without rinsing to methyl blue solution for 5 minutes
8. Rinse in water
9. Dehydrate, clear and mount
Hazard Information
UN1170
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
