Luxol Fast Blue Stain Kit
CE / IVD
This stain kit is used to demonstrate the presence of normal myelin. When combined with a cresyl violet counterstain myelin and nissl substances are demonstrated
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK345 | 5 x 50ml | 5 x 100ml | 5 x 250ml | 5 x 500ml |
Kit Contains:
Luxol Fast Blue 0.1% in Acidified Methanol
Cresyl Violet 0.5% Aqueous Solution
Cresyl Violet Differentiator (IMS 99%)
Lithium Carbonate Solution 0.05%
Denatured Ethanol 70%
General Information:
Procedure Time : 140 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Myelin: Blue/Green
Nuclei, Nissl Substances: Violet/Pink
Protocol:
1. Dewax sections and hydrate to 95% alcohol. Do not rinse.
2. Stain in luxol fast blue solution for 2 hours at 60 ° C or at 37°C overnight
3. Wash in 70% denatured ethanol for 2-3 seconds to remove excess stain
4. Wash in tap water
5. Differentiate using lithium carbonate solution until the grey and white matter are distinguished
6. Wash in tap water
7. Check differentiation under the microscope. Repeat step 5 if necessary
8. Stain in cresyl violet solution for 10-12 minutes
9. Wash in tap water
10. Differentiate in cresyl violet differentiator for 4-8 seconds
11. Check differentiation under microscope (only look at nissl substances and nuclei)
12. Dehydrate, clear and mount
Hazard Information
UN1992
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
