Martius Scarlet Blue Stain Kit (MSB)

SKU: RRSK2 Categories: , ,

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CE / IVD 
This stain kit allows the differentiation of fibrin, collagen and muscle. Early fibrin and red blood cells are also demonstrated with this technique

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Description

100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK26 x 50ml6 x 100ml6 x 250ml6 x 500ml

 

Kit Contains:
Martius Yellow Sat. Alcoholic with 2% Phosphotungstic Acid
Phosphotungstic Acid 1% Solution
Haematoxylin Weigerts (Solution A)
Brilliant Crystal Scarlet
Aniline Blue (1% aqueous)
Haematoxylin Weigerts (Solution B)

General Information:
Procedure Time : 45 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:    15-25° C.

Results: 
Fibrin:                                                                                  Red (early fibrin may stain yellow and old fibrin blue)
Muscle:                                                                                Pale red
Nuclei:                                                                                  Blue/black
Collagen; basement membranes; reticulin:                  Blue
Elastic fibres:                                                                      Blue
Erythrocytes:                                                                      Yellow

 

Protocol:  

1. Prepare fresh Weigert haematoxylin by mixing equal volumes of solution A & B as required
2. Dewax sections, hydrate through alcohols and rinse in tap water
3. Stain nuclei with Weigerts iron haematoxylin for 10 minutes
4. Wash quickly in water and differentiate in 1% acid alcohol solution (RRSP187 – not supplied), leaving the nuclei slightly overstained
5. Wash well in running tap water and then Blue in water or Scotts tap water
6. Wash well in running water
7. Rinse in 95% alcohol (not supplied)
8. Stain with martius yellow solution for 5 minutes
9. Wash quickly in running tap water
10. Stain with crystal scarlet solution for 5 minutes
11. Wash in running tap water
12. Treat with phosphotungstic acid solution for 10 minutes, check microscopically. Treat for a further 5 minutes if necessary
13. Wash in running tap water
14. Stain with aniline blue solution for 5 minutes
15. Wash in tap water
16. Dehydrate, clear and mount

 

Hazard Information

 

UN1170

 

 

 

Additional information

Pack

100 Test, 1000 Test, 200 Test, 500 Test