Reticulin Stain Kit (Gordon & Sweets 1936) (with Gold Chloride)

Description

Kit Contains:
Potassium Permanganate 0.5% Solution x 2
Sulphuric Acid 3% Solution
Oxalic Acid 1% Solution
Ferric Ammonium Sulphate 2.5% Solution
Silver Nitrate 10% Solution
Ammonia 33% Solution
Sodium Hydroxide 3% Solution
Formaldehyde 10% (v/v) Solution Unbuffered
Sodium Thiosulphate 5% Solution
Gold Chloride 0.2% Solution
Neutral Red 0.5%

General Information:
Procedure Time : 50 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Store silver nitrate 2-8°C. Other reagents 15-25 °C

Results: 
Reticulin fibres:    Black
Nuclei:                    Black (lipofuchsin and melanin are also weakly stained)
Other Elements:    Red

Protocol:  

1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise the sections with acidified potassium permanganate for 5 minutes
3. Wash well in tap water
4. Decolourise with oxalic acid solution for 1 minute
5. Wash sections well in tap water
6. Mordant sections in ferric ammonium sulphate solution for 15 minutes
7. Wash well with several changes of distilled water
8. Treat with filtered ammoniacal silver solution for 12-15 seconds
9. Wash well in several changes of distilled water (the section must be transluscent)
10. Reduce in the formaldehyde solution for 1-2 minutes, check microscopically and if under impregnated repeat steps 8-10
11. Wash in distilled water
12. Tone in 0.2% gold dhloride solution for 3-5 minutes
13. Wash in distilled water. Treat with sodium thiosulphate for 5 minutes
14. Wash well in water
15. If counterstain is required stain with neutral red for 30 seconds
16. Rinse in water
17. Dehydrate through alcohols, clear and mount

Hazard Information

UN3082