Reticulin Stain Kit (without Gold Chloride)
CE / IVD
This stain kit is used to demonstrate reticulin fibres. The Gordon & Sweet method uses metal impregnation providing excellent contrast so even the finest fibres can be seen
Description
| 100 Test | 200 Test | |
| Code | – 100 | -200 |
| RRSK102 | 11 x 50ml | 11 x 100ml |
Kit Contains:
Potassium Permanganate 0.5% Solution x 2
Sulphuric Acid 3% Solution
Oxalic Acid 1% Solution
Ferric Ammonium Sulphate 2.5% Solution
Silver Nitrate 10% Solution
Ammonia 33% Solution
Sodium Hydroxide 3% Solution
Formaldehyde 10% (v/v) Solution Unbuffered
Sodium Thiosulphate 5% Solution
Neutral Red 0.5%
General Information:
Procedure Time : 50 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: Store silver nitrate 2-8°C. Other reagents 15-25 °C
Results:
Reticulin fibres: Black
Nuclei: Black (lipofuchsin and melanin are also weakly stained)
Other Elements: Red
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise the sections with acidified potassium permanganate for 5 minutes
3. Wash well in tap water
4. Decolourise with oxalic acid solution for 1 minute
5. Wash sections well in tap water
6. Mordant sections in ferric ammonium sulphate solution for 15 minutes
7. Wash well with several changes of distilled water
8. Treat with filtered ammoniacal silver solution for 12-15 seconds
9. Wash well in several changes of distilled water (the section must be transluscent)
10. Reduce in the formaldehyde solution for 1-2 minutes, check microscopically and if under impregnated repeat steps 8-10
11. Wash in distilled water
12. Treat with sodium thiosulphate for 5 minutes
13. Wash well in water
14. If counterstain is required stain with neutral red for 30 seconds
15. Rinse in water
16. Dehydrate, clear and mount
Hazard Information
UN3316
Additional information
| Pack | 100 Test, 200 Test |
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