Gram Stain Kit (Twort Counterstain)
CE / IVD
This stain kit is used to demonstrate Gram Positive and Gram Negative Micro-organisms. The main principle of the technique is the increased thickness, chemical composition and functional integrity of the cell walls of gram positive bacteria, which when they die become gram negative
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK45 | 5 x 50ml | 5 x 100ml | 5 x 250ml | 5 x 500ml |
Kit Contains:
Crystal Violet (0.5% Aqueous)
Grams Iodine
Gram Differentiator x 2
Twort Solution A ( 0.2% Neutral Red in IMS)
Twort Solution B (0.2% Fast Green in IMS)
General Information:
Procedure Time : 18 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Gram Positive Organisms: Blue/Black
Gram Negative Organisms: Red/Pink
Nuclei: Red/Pink
Red Blood Cells and most cytoplasmic structures: Green
Elastic Fibres: Black
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water. For smears flood slides with distilled water
2. Stain with crystal violet for 3 minutes (filter before use)
3. Rinse well in distilled water
4. Treat slides with Grams iodine for 3 minutes
5. Wash well in distilled water, drain
6. Differentiate with Grams differentiator until no more colour comes out of the preparation. (1-5 seconds) Rinse off quickly, sections will be straw coloured
7. Immediately wash well in water
8. Mix Twort stain A&B (Twort working solution) immediately before use, as follows: solution A (0.2% neutral red) 9 parts, solution B (0.2% fast green) 1 part, distilled water 30 parts. Stain with the working solution for 3 minutes
9. Wash in water, blot dry and allow to air dry
10. Dehydrate rapidly, clear and mount
Hazard Information
UN1993
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
