Gram Stain Kit (Hucker & Conn)

SKU: RRSK200 Categories: , ,

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This stain kit is used to demonstrate Gram Positive and Gram Negative Micro-organisms. The main principle of the technique is the increased thickness, chemical composition and functional integrity of the cell walls of gram positive bacteria, which when they die become gram negative

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK2005 x 50ml5 x 100ml5 x 250ml5 x 500ml


Kit Contains:
Crystal Violet Hucker & Conn
Safranin 0.5% aqueous
Grams Iodine
Gram Differentiator x 2

General Information:
Procedure Time : 20 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:    15-25° C.

Gram Positive and Fungi:  Dark Blue/Violet
Gram Negative Organisms:  Red


1. Dewax sections, hydrate through alcohols and rinse in tap water. For smears flood slides with distilled water
2. Apply crystal violet solution and leave for 2-3 minutes
3. Wash with water
4. Apply Grams iodine and leave for 2-3 minutes
5. Apply several changes of Grams differentiator until no more colour appears to flow from preparation
6. Wash well in water
7. Apply Safranin for 4 minutes
8. Wash in water and blot dry . For smears allow to dry
9. Dehydrate, clear and mount

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100 Test, 1000 Test, 200 Test, 500 Test