Gram Stain Kit (Hucker & Conn)
CE / IVD
This stain kit is used to demonstrate Gram Positive and Gram Negative Micro-organisms. The main principle of the technique is the increased thickness, chemical composition and functional integrity of the cell walls of gram positive bacteria, which when they die become gram negative
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK200 | 5 x 50ml | 5 x 100ml | 5 x 250ml | 5 x 500ml |
Kit Contains:
Crystal Violet Hucker & Conn
Safranin 0.5% aqueous
Grams Iodine
Gram Differentiator x 2
General Information:
Procedure Time : 20 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Gram Positive and Fungi: Dark Blue/Violet
Gram Negative Organisms: Red
Protocol:
1. Dewax sections, hydrate through alcohols and rinse in tap water. For smears flood slides with distilled water
2. Apply crystal violet solution and leave for 2-3 minutes
3. Wash with water
4. Apply Grams iodine and leave for 2-3 minutes
5. Apply several changes of Grams differentiator until no more colour appears to flow from preparation
6. Wash well in water
7. Apply Safranin for 4 minutes
8. Wash in water and blot dry . For smears allow to dry
9. Dehydrate, clear and mount
Hazard Information
UN1993
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
