Wheatleys Trichrome Stain Kit
CE / IVD
This stain kit is commonly used for the staining of foecal films to aid in the diagnosis of intestinal protozoa
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK63 | 4 x 50ml | 4 x 100ml | 4 x 250ml | 4 x 500ml |
Kit Contains:
Wheatley’s Trichrome Stain
Wheatley’s Differentiator
Denatured Ethanol 70%
Iodine 2% Alcoholic
General Information:
Procedure Time : 55 minute (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25 °C
Results:
Cytoplasm of Protozoan trophozoites: Blue/Green
Cysts: Purple
Nuclei and inclusions (chromatoid bodies, RBC, bacteria, and Charcot-Leyden crystals): Red, sometimes tinged with purple
Protocol:
1. Remove slide from Schaudinn’s fixative, and place slide in 70% denatured ethanol for 5 min
2. Place slide in Iodine 2% alcoholic solution for 1 min for fresh specimens or 5-10 min for PVA-preserved air-dried smears
3. Place slide in 70% denatured ethanol for 5 min
4. Place slide in 70% denatured ethanol again for 3 min
5. Place in trichrome stain for 10 min
6. Place in Wheatley’s differentiator for 1-3 seconds. Immediately drain the rack, and proceed to the next step. Do not allow slides to remain in this solution
7. Dip several times in 100% ethanol (not supplied). Use this step as a rinse
8. Place in two changes of 100% ethanol (not supplied) for 3 min each
9. Place in xylene for 5 min
10. Place in xylene again for 5 min
11. Mount with coverslip (no. 1 thickness) by using mounting medium
12. Allow the smear to dry overnight or after 1 hr at 37°C
13. Examine the smear microscopically with 100X objective. Examine at least 200-300 oil immersion fields
Hazard Information
UN1170
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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