Orcein Shikata Stain Kit (for HbAg in tissue)
CE / IVD
This stain kit is a popular for staining hepatitis B surface antigen and copper associated protein commonly found in diseases such as Wilson’s disease and chronic biliary disease
Description
100 Test | 200 Test | 500 Test | 1000 Test | |
Code | – 100 | -200 | -500 | -1000 |
RRSK29 | 6 x 50ml | 6 x 100ml | 6 x 250ml | 6 x 500ml |
Kit Contains:
Orcein solution (1% Orcein in 1% acid alcohol)
Potassium Permanganate 0.5% solution x 2
Sulphuric Acid 3% Solution
Oxalic Acid 1% Solution
Denatured Ethanol 70%
General Information:
Procedure Time : 140 minutes (post warming of working solution) (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Hepatitis B surface antigen: Brown granules in cytoplasm
Elastic Fibres: Brown
Copper associated protein: Dark Brown/Purple
Background: Pale Pink/Brown
Protocol:
Preparation of Acidified Potassium Permanganate
Mix 9.5ml of potassium permanganate solution with 0.5ml of sulphuric acid solution. This purple solution will keep for 3-4 weeks but should be discarded after using 5 times or if it turns brown
1. Dewax sections, hydrate through alcohols and rinse in tap water
2. Oxidise sections with acidified potassium permanganate for 5 minutes
3. Wash well in water
4. Bleach with 1% oxalic acid for 1 minute
5. Wash well in water
6. Rinse sections in 70% denatured ethanol
7. Stain in orcein solution for 2 hours at 56°C
8. Rinse in water then 70% denatured ethanol
9. Dehydrate, clear and mount
Hazard Information
UN1170
Additional information
Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
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