Oil red O Stain Kit

SKU: RRSK14 Categories: , ,

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This stain kit is used to demonstrate hydrophobic lipids especially neutral fats in tissue. In order to penetrate the fats the dye must be dissolved in organic solvents, the solvent must be sufficiently dilute (aqueous) to avoid extracting the lipids into the solution. The most widely used solvent is isopropyl alcohol (isopropanol/IPA). Also, the tissue once stained, can be  shown by polarised light to demonstrate birefringement cholesterol

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100 Test200 Test500 Test1000 Test
Code– 100-200-500-1000
RRSK144 x 50ml4 x 100ml4 x 250ml4 x 500ml


Kit Contains:
Saturated Oil Red O Stain in Isopropyl Alcohol
Haemalum Mayer
Isopropyl Alcohol 70% x 2

General Information:
Procedure Time :  20 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage:    15-25° C.

Unsaturated hydrophobic lipids (ie cerebrosides, triglycerides, chlolesterol esters):    Red
Phospholipids:                                                                                                                                Pink
Nuclei:                                                                                                                                              Blue

Working Stock Solution 
Working Solution: Add 4 parts distilled water to 6 parts oil red O stain. Mix well and stand for 10 minutes,
filter before use. Use immediately after filtration


1. Wash slides with distilled water
2. Rinse with 70% isopropyl alcohol to clear background
3. Stain with freshly prepared oil red O working solution 15 mins
4. Rinse briefly with 70% isopropyl alcohol. Carefully rinse in distilled water
5. Lightly stain nuclei with alum haematoxylin for 10 seconds
6. Rinse with distilled water and blue
7. Mount in aqueous mountant or examine under immersion oil

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100 Test, 1000 Test, 200 Test, 500 Test