Oil red O Stain Kit
CE / IVD
This stain kit is used to demonstrate hydrophobic lipids especially neutral fats in tissue. In order to penetrate the fats the dye must be dissolved in organic solvents, the solvent must be sufficiently dilute (aqueous) to avoid extracting the lipids into the solution. The most widely used solvent is isopropyl alcohol (isopropanol/IPA). Also, the tissue once stained, can be shown by polarised light to demonstrate birefringement cholesterol
Description
| 100 Test | 200 Test | 500 Test | 1000 Test | |
| Code | – 100 | -200 | -500 | -1000 |
| RRSK14 | 4 x 50ml | 4 x 100ml | 4 x 250ml | 4 x 500ml |
Kit Contains:
Saturated Oil Red O Stain in Isopropyl Alcohol
Haemalum Mayer
Isopropyl Alcohol 70% x 2
General Information:
Procedure Time : 20 minutes (approximate)
Shelf Life: 3 Years (from date of manufacture)
Storage: 15-25° C.
Results:
Unsaturated hydrophobic lipids (ie cerebrosides, triglycerides, chlolesterol esters): Red
Phospholipids: Pink
Nuclei: Blue
Working Stock Solution
Working Solution: Add 4 parts distilled water to 6 parts oil red O stain. Mix well and stand for 10 minutes,
filter before use. Use immediately after filtration
Protocol:
1. Wash slides with distilled water
2. Rinse with 70% isopropyl alcohol to clear background
3. Stain with freshly prepared oil red O working solution 15 mins
4. Rinse briefly with 70% isopropyl alcohol. Carefully rinse in distilled water
5. Lightly stain nuclei with alum haematoxylin for 10 seconds
6. Rinse with distilled water and blue
7. Mount in aqueous mountant or examine under immersion oil
Hazard Information
UN1219
Additional information
| Pack | 100 Test, 1000 Test, 200 Test, 500 Test |
|---|
